TBE Buffer

TBE buffer contains Tris hydroxymethyl aminomethane, Boric acid, and Ethylenediaminetetraacetic acid (EDTA)

Step1: Making a 10X stock solution
Step 2: Dilution

Step 1: Making a 10X stock solution

The composition is as follows:

1. Tris: 108 g
2. Boric acid: 55 g
3. EDTA: 186 g or Liquid EDTA 0.5M, pH 8.0: 40 ml 
4. SDW: Up to the volume 1000 ml

-----------------------------------------------------------------

Step 2: Dilution of 10X buffer to 0.5X 

The common and easy formula to calculate the dilution is- 

C1V1 = C2V2

Here,

• C1 = 10
• C2 = 0.5
• V2 = 1000 (suppose 1 liter)
• V1 = ?

                                                         10 X V1 = 0.5 X 1000

                                                    or, V1 = (0.5 X 1000) / 10

                                                    or, V1 = 50

So, we need to take 50 ml from the 10X stock buffer and dilute with 950 ml SDW